FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6309096
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG11550.017.575338145
CGTGTAG353000.011.028773145
AGTGTAG143650.010.850768145
CGCCGTA17400.010.833075145
CGTATCA17500.010.771172145
TAGGGAA287350.010.5965805145
TGGTCGC34900.010.594303145
GCCGTAT17950.010.5011425145
GAGTGTA158150.010.36017145
GTGTAGG341600.010.229557145
AGAGTGT183800.010.097691145
TCGCCGT30000.09.908097145
TGTAGGG324450.09.898824145
TAGATCT115500.09.854743145
TCGTGTA373600.09.760875145
GTGTAGA140750.09.683605145
GTCGTGT400500.09.61213145
AGATCTC119700.09.569529145
AGCGTCG491900.09.565232145
CGTCGTG435900.09.463528145
Produced by FastQC (version 0.11.5)