FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7439918
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG23650.031.574707145
CGCCGTA54550.015.682689145
GGATGGG121000.014.5005243
CGTATCA43150.012.76927145
GCCGTAT49400.012.768079145
AGGATGG136650.012.4685172
GTGGTCG108750.012.399864145
ATGGGGG88950.012.3067635
GAGGATG139800.011.9418311
CCGTATC49800.011.937621145
TGGTCGC93400.011.1776085145
TCGCCGT75900.010.602651145
AGAGCGG54000.010.203592145
TCTCGGT176400.010.069334145
GTCGCCG90500.09.853483145
AGTGTAG311350.09.7565975145
GTAGATC309400.09.419742145
GAGTGTA330950.09.332124145
AGATCTC244950.09.32323145
TGTAGAT312450.09.234977145
Produced by FastQC (version 0.11.5)