FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058291
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68430.33246027894014984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67040.32570710361168564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59310.2881516753461974No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45090.21906523421615312No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42460.2062876434867567No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34100.16567142352563363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.032.0063481
CGTTCGG602.423567E-729.99575824
GCAGCGT7300.028.5107571
AGCGTCA7650.027.9406173
CAGCGTC8500.025.1551132
TATCGGT500.002267076622.49681724
TTCGGTA754.609926E-520.9970326
CGCGGTA4250.020.11480343
CGCTATA807.5481104E-519.6938042
TATACCG807.573527E-519.6851926
CGGTAAG1151.5992555E-719.5624528
CTCTAAT6550.018.9961341
CCGAACG600.006517525318.74734913
GCGTCAG11650.018.3472734
GTATCAA27700.018.0382652
TCGTTCG901.8686021E-417.49752423
AATCGTC901.8686021E-417.49752428
CGGTACC5050.017.3737845
GTGTATA13000.017.30524313
TAACGGC4850.017.16252136
Produced by FastQC (version 0.11.5)