FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689769
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1628427
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19650.12066859613602575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.028.4421771
TTAGACT1151.3460522E-1020.9130444
GTTAGAC450.003826482720.5555553
ATTCGCG801.6173452E-518.530
CGTAGCC1750.017.971433
GTATCAA11400.017.8508762
GGTCTAG1351.1532393E-917.8148141
GTCGAAA1708.54925E-1116.3235322
TTTAGAC1151.2428955E-616.0869563
TTAGACA1403.475725E-815.8571434
CGGTCGA1802.0190782E-1015.41666620
TTCGCGG1802.0190782E-1015.41666631
TCTATAC1455.349284E-815.3103453
CTATACA1601.0972144E-815.031254
ACTACAC2600.014.9423073
TATACTG1752.239176E-914.85
AATTCGC1001.0938086E-414.79999929
GTATTAG5050.014.6534641
CGCGCTT1904.5656634E-1014.60526412
AGACTAT1405.998754E-714.5357156
Produced by FastQC (version 0.11.5)