FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689772
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3951095
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT86800.21968593516480875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73240.18536633515519116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50580.1280151451686178No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC43510.11012137141729066No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG39960.10113652038232439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24050.028.8461531
GTATCAA38000.018.159212
TCGCTAA1803.3378456E-914.38888914
ATTAGAG16000.014.2218753
GTATTAG20150.014.1389581
TATCGGA2252.7284841E-1113.97777726
CTAATAC17000.013.8205873
TAACGGC12900.013.76744236
TATTAGA17350.013.7550422
TTAACGG12950.013.71428635
TAATACT18450.013.6368554
TCTAATA15500.013.6064512
ATACTGG17900.013.4357556
TTAGACT3600.013.3611124
GCACCGC16000.013.18125110
CTCTAAT13500.013.0185191
CGCGAAC1000.00183385512.95000128
GTCGCCC12750.012.91372637
TTTAACG14500.012.88620834
TCTTATA41100.012.87347937
Produced by FastQC (version 0.11.5)