FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3467299
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96950.27961245915047994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95780.27623807465119105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88430.25504001818129907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61060.1761024936124632No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53610.15461602821100806No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45350.13079345046389135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.032.990381
AGCGTCA10650.028.5197373
GCAGCGT12250.024.7409231
CAGCGTC14100.022.186172
GCGTCAG16200.018.7490864
GTATCAA39350.018.7020682
CGTCAGA18650.016.284665
GTGTATA19750.015.9472113
TATAAGA20850.015.3216716
TAACGGC5000.015.29837236
ATAAGAG21000.015.21222817
GTACCGA900.003759336414.9979726
TGTATAA21050.014.74859914
GTCAGAT21100.014.5004086
ATGTGTA22600.014.43388811
CGCCGTA1251.3833125E-414.39805325
TATATCG1758.1947655E-714.14094455
CGGGGTA5750.014.085052545
GTATAAG22150.014.01616215
GTTCGCG2103.0007868E-813.92668830
Produced by FastQC (version 0.11.5)