FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609246
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102180.28310622218601894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94440.26166129989477027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87780.2432086923418354No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69470.19247787488023815No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69360.1921731020828173No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53360.14784251336705784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.031.2499641
AGCGTCA14750.029.8967863
GCAGCGT16550.025.5690381
CAGCGTC17550.025.1345882
GCGTCAG20350.022.0013394
CGTCAGA24400.018.7164975
GTGTATA24400.018.347713
GTATCAA40150.018.0357931
ATAAGAG26550.017.0313817
GTATAAG26700.016.85144215
GTCAGAT26800.016.7885636
ATGTGTA27650.016.6792711
TGTATAA27400.016.33882514
TATAAGA27800.016.02281216
TGTGTAT29400.015.68645812
TAAGAGA30200.015.41990718
GATGTGT30100.015.17217610
AAGAGAC30300.014.84929119
TCAGATG30600.014.7772277
CAGATGT31050.014.6355178
Produced by FastQC (version 0.11.5)