FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005689778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005689778
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2184022
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48210.22073953467501703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41300.18910065924244351No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27980.12811226260541334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13300.029.071431
GTCGAAA2000.022.222
CGGTCGA2050.021.65853520
TCGAAAT2300.020.10869423
TCGCGGT2650.018.84905617
GTATCAA20850.018.5443652
ATCGCGG2900.017.22413816
CGAAATA2600.017.07692124
TATACTG2550.015.2352945
CGCGCTT855.366899E-415.23529312
CATCGCG3350.014.91044715
TCTATAC1406.0014827E-714.5357143
TGTACCG1304.4480876E-614.2307685
GCCTAAG5600.014.2053571
AAGACCG1601.7854836E-713.8755
CTCTTAT35100.013.54558437
CGAACTA5250.013.39047524
GTCTAGA1409.56744E-613.2142851
ATCAACG29750.013.1831934
TATGCCG3800.013.1447375
Produced by FastQC (version 0.11.5)