FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31445305
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17650.018.44344748
TCGTATG18300.017.78812645
CGTATGC20200.016.11503846
CGACTAA15600.015.929255528
TCGATAG85600.015.0886745
CGATAGG88200.014.64392546
CTCGATA91800.014.03185944
CCGACTA20200.012.64780827
CTCGTAT25300.012.31344444
GTGCTCG109500.012.24319541
GCGGGTT54300.011.54186251
TGCCGTG118400.011.53007137
GTCGGAT17400.011.2683791
GCCAGAT227700.011.1326421
CCGTGCT123450.011.0339
TGCTCGA126450.010.98968442
GGATACC122300.010.67543567
TAATACG50500.010.5329926
GTACGAT85500.010.52131866
GCTCGAT133800.010.43809743
Produced by FastQC (version 0.11.5)