FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31780208
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA20600.022.76676228
CCGACTA24050.019.64575427
TATGCCG17250.019.27668648
TCGTATG18200.018.27040145
CGTATGC20900.016.07748246
ACAACCG31950.014.89674723
CGATAGG73250.014.76535846
TCGATAG74050.014.70046845
CTCGATA81900.013.37721944
CTCGTAT26400.013.12612844
GCGGGTT61200.012.0143351
GTCGAAT17650.011.704131
GTGCTCG97950.011.39983341
TGCCGTG101850.010.894737
CCGTGCT104700.010.73193739
TGCTCGA107450.010.52217742
GCCAGAT230850.010.4804531
GTCGCGA6058.5310603E-1010.4171511
TCGCGTG43050.010.3233949
GGATACC106600.010.27760367