FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29995661
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAA15550.023.41097536
CGACTAA19550.022.0200228
CGCAATC21200.017.00653338
CCGACTA25550.016.71166227
TCGATAG85150.015.70322245
CGATAGG85500.015.5981346
CTCGATA88750.015.22405944
TATGCCG14300.014.93171548
TCTACGC25100.014.92158734
ACAACCG29100.014.91267223
ACGCAAT26600.014.343636537
GTGCTCG104750.013.16595241
AATCGCG4902.1827873E-1112.85838741
TGCCGTG109050.012.8074237
TGCTCGA117300.012.05572742
CGCGTAA3802.1561937E-711.97631
CGTATGC18900.011.85309846
GCCAGAT231900.011.8201761
CCGTGCT118150.011.73212239
ATGCGTA39700.011.72655657
Produced by FastQC (version 0.11.5)