FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35818510
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG17550.024.9254536
CCGACTA23200.023.23215927
CGACTAA24400.022.66417128
TATGCCG26250.022.00115248
CGTATGC32100.018.53684646
ACGTCGT34100.018.47534632
TCGTATG32700.018.19654545
ATACGGC26050.017.1952115
ACAACCG34450.015.74617423
CGATAGG81950.014.47913746
TCGATAG83650.014.059216545
ACCGAGA30900.014.04310216
CTCGATA88300.013.39845444
TGATACG34150.013.3215913
CTCGTAT44650.013.01323644
GCGGGTT73300.012.6109321
ACCGACT45950.012.33905926
GTGCTCG98450.012.19512341
TCGGTTC40100.011.868563
GTATGCC51750.011.49815547
Produced by FastQC (version 0.11.5)