FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61483275
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA35550.021.85594428
CCGACTA47650.015.86502227
TCGATAG131600.014.62846245
CGATAGG132300.014.28647646
TATGCCG27100.014.0780848
CTCGATA145100.013.31562244
ACAACCG59800.012.75840323
ATGCGTA71800.011.9440157
TCGTATG32000.011.70376845
GTGCTCG171200.011.44914241
GTCGGAT35300.011.1078471
GCCAGAT430900.010.927791
TGCCGTG185250.010.82623337
GCGGGTT108550.010.5464061
GCCGATT50700.010.3578561
TGCTCGA196700.010.33862942
CGTATGC35950.010.32043946
CCGTGCT192800.010.2570839
GTCGAAT35600.010.0308271
AGCGAAC135800.09.76870561
Produced by FastQC (version 0.11.5)