FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34316706
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA19150.018.09345428
CGATAGG93400.016.11420846
CCGACTA21800.016.05400727
TCGATAG96950.015.63251145
TATGCCG16350.015.41371148
CTCGATA102800.014.77733244
CGTATGC19250.013.27331846
TCGTATG19500.013.10318545
GTGCTCG119650.012.842978541
GTCGGAT18950.012.3796411
TGCTCGA128900.012.27432442
TGCCGTG129550.012.05097437
CCGTGCT131500.012.00531639
ACAACCG30700.011.74136423
GGATACC135950.011.30263367
GCCAGAT275900.011.1044951
GCTCGAT145100.010.87964843
GATAGGG148550.010.48519847
ATGCCGT162650.010.45931236
CGGGCTA20000.010.3291471
Produced by FastQC (version 0.11.5)