FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33783482
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28350.020.98841948
TCGTATG29950.020.21764645
CGTATGC32350.019.36701246
CGACTAA18300.018.55151728
TATCTCG36650.016.1405841
TCGATAG96850.015.79294545
CGATAGG97500.015.61595446
CTCGTAT39000.015.16760344
CTCGATA101850.014.94937544
CCGACTA24800.013.83018727
GTATGCC51450.012.72153447
GTGCTCG121900.012.66313641
TGCCGTG133900.011.78993437
TGCTCGA135150.011.68057742
CCGTGCT134500.011.58122339
GTACGAT84500.011.47455566
GCCAGAT263400.011.4053441
GGATACC139050.011.07622967
ACAACCG32250.011.06879423
AGTACGA86900.010.99618865
Produced by FastQC (version 0.11.5)