FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31490587
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA16650.019.9698228
TATGCCG14450.017.4407848
CCGACTA19700.016.69974527
CGATAGG80450.014.35768346
TCGATAG81450.014.35328145
TCGTATG17100.014.32840145
CTCGATA85800.013.50342944
CGTATGC19800.013.25844546
ACAACCG27050.012.80819323
ATGCGTA36350.012.13313357
GTGCTCG105300.011.33545741
GTCGAAT17950.011.1177421
TGCCGTG113450.011.015223537
GTCGATT23000.010.80551934
TGCTCGA114600.010.7821842
CCGTGCT112900.010.75867839
GCCAGAT231900.010.6739431
CGGGCTA17250.010.5540821
CGCGTAT5006.132541E-810.5033391
ACGTCGA25700.010.35080632
Produced by FastQC (version 0.11.5)