FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31511342
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17300.021.0430548
TCGTATG17650.020.03024945
CGACTAA18750.017.73292428
CGTATGC21650.017.29988346
TCGATAG73850.016.63666745
CGATAGG74750.016.3429946
CTCGATA81900.015.17263644
CCGACTA21700.015.1601727
GTGCTCG97700.012.68283541
ATGCGTA35850.012.49761357
TGCCGTG104650.011.97460237
GCCAGAT224300.011.862421
CGACCGT12750.011.8015693
TGCTCGA109600.011.59328442
GCGGGTT54750.011.5739981
GTACGAT74650.011.44149566
CCGTGCT112850.011.38345739
AGTACGA76150.011.3540665
GGATACC112750.011.05230367
AGTACGT79500.010.78788351
Produced by FastQC (version 0.11.5)