FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33088611
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18300.028.69002748
CGTATGC22700.024.2081746
TCGTATG22350.023.80416345
CGACTAA20650.021.0169728
CCGACTA22300.019.30433827
CGATAGG97350.016.93452846
TCGATAG99550.016.59539445
CTCGATA103150.015.98261144
CTCGTAT36500.014.57628244
ACAACCG31350.014.28894623
GTATGCC39200.013.92921747
GTGCTCG118800.013.90704141
TGCCGTG126400.013.62481137
TGCTCGA130950.013.17789342
CCGTGCT130950.012.88407739
GCGGGTT64100.012.1812141
GGATACC139050.011.88156467
GTACGAT84800.011.68130266
AGTACGA87100.011.65402365
ACGTCTG50400.011.45661415
Produced by FastQC (version 0.11.5)