FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37040746
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20700.023.5047848
TACGATC20000.021.17693338
CGACTAA24750.021.0709228
ATACGAT22400.020.93935437
CGAATAC23950.020.0231634
CCGACTA25900.019.99969127
TCGTATG24700.019.55649445
CGTATGC25850.018.8219646
ACCGAAT25950.018.61443932
CCGAATA28950.016.68564233
GAATACG28900.016.5935935
TCGATAG101650.015.59910345
CGATAGG102550.015.49639846
CTCGATA106800.014.94546644
ACAACCG35700.014.31186323
AATACGA39350.012.63151436
GTGCTCG130700.012.23883441
CGCGATT6050.012.1526581
CTCGTAT38550.011.80415444
TGCCGTG137200.011.73573337
Produced by FastQC (version 0.11.5)