FastQCFastQC Report
Mon 13 May 2024
EGAF00005706900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57022833
Total Bases8.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC38498886.751484970941377TruSeq Adapter, Index 2 (100% over 50bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC806070.14135916396858078TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGC659160.11559580001926596TruSeq Adapter, Index 2 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)