FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57022833
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC38498886.751484970941377TruSeq Adapter, Index 2 (100% over 50bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC806070.14135916396858078TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGC659160.11559580001926596TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA7192050.084.8653269
AGAGCAC7521150.081.207798
AAGAGCA7585450.080.625467
CGGAAGA7662500.079.816064
TCGGAAG7778200.078.646513
GATCGGA7828900.078.015381
ATCGGAA7868700.077.805442
GAAGAGC8076750.075.826446
GGAAGAG8545100.071.673845
TATGCCG4566000.027.20734445-49
TCGTATG4650550.026.76240240-44
CGTATGC4626800.026.6739240-44
CTCGTAT4716250.026.33949340-44
GTATGCC4654350.026.1888945-49
TATCTCG4543850.025.92769635-39
TCTCGTA4636950.025.6271940-44
GCCGTCT4818500.025.4934545-49
CCGTCTT4776350.025.3946650-54
CGTCTTC4901300.025.27604750-54
TGCCGTC4942850.025.16322145-49
Produced by FastQC (version 0.11.5)