FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33887214
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA20550.019.58601228
TATGCCG17050.018.06546248
TCGTATG18550.017.73669845
CCGACTA24150.016.81078727
TCGATAG64400.015.81597545
CGATAGG66150.015.34464946
CGTATGC21700.014.83942146
CTCGATA72650.014.16489644
ACGACGT24250.013.27896732
ATGCGTA41850.012.21136557
ACAACCG33750.012.13208823
CGACGTC28800.011.78903833
TATCTCG28150.011.68853541
GTGCTCG91800.011.62968141
GCCAGAT231700.011.2424441
TGCTCGA100050.010.77568542
TGCCGTG98500.010.7677637
GCGGGTT60750.010.6620151
GTCGAAT19850.010.4065161
CCGACCG14800.010.4039372
Produced by FastQC (version 0.11.5)