FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34515427
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA20550.018.39398628
TCGATAG85600.014.63834945
TCGTATG16800.014.5838345
CGATAGG85950.014.53797346
TATGCCG17600.014.11998348
GTCGAAT19700.014.0414631
CCGACTA27000.013.86985827
CTCGATA95450.013.20140344
CGTATGC20100.012.537703546
GCCGATT27800.011.9654851
GTCGCAT17650.011.9030441
GTGCTCG113700.011.42155541
GTCGGAT17900.011.14996051
GCCAGAT243750.011.1472711
GTCGATT16650.010.725251
CGCGATT5301.2714736E-810.5705011
TATCTCG28650.010.50716541
CTCGTAT25650.010.37097844
TGCCGTG129650.010.25970337
ACAACCG35400.010.18289923
Produced by FastQC (version 0.11.5)