FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005706912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005706912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31986108
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17750.023.4665848
TCGTATG20000.021.17615345
CGTATGC23050.019.4372546
CGACTAA19900.018.1151228
CCGACTA22900.015.1304127
TCGATAG75300.014.22388245
CGATAGG75600.014.16757146
CTCGATA82150.013.29370944
ACGAGAG33050.013.13204732
CTCGTAT31750.012.898561544
ACAACCG29000.012.67135923
GTCGAAT17150.011.2279811
GTGCTCG98050.011.20956341
GCCAGAT221600.010.9646011
CGGGCTA17250.010.95992851
GTATGCC40950.010.9409247
CCGTGCT109400.010.3667339
TGCCGTG108900.010.28577137
TGCTCGA113200.010.11143342
TCGCGTG45550.09.9106019
Produced by FastQC (version 0.11.5)