FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75297239
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAAT2002507726.594702894750235No Hit
CGGAGGGA1675122722.24680110780689No Hit
GTCTCTCG1580069220.984424143360688No Hit
AATCTCTC1545701520.52799704913483No Hit
AATCCCTC12691851.6855664521776157No Hit
GTCTCCCG7253600.9633288147524242No Hit
GTCCCTCG5501920.7306934587601546No Hit
AATCTCCC4606260.6117435461345402No Hit
TCAGAAAC3387760.44991822342914856No Hit
GCCTCTCG2607890.34634603268786524No Hit
TCAGAAAG2381370.31626259231098763No Hit
TCAGAAAA2327380.30909234268204705No Hit
GGCTCTCG2031610.26981201794132187No Hit
CCAGAAAT1964330.26087676335648907No Hit
AATCGCTC1784590.23700603417875654No Hit
NCAGAAAT1729800.22972953895427692No Hit
AATCACTC1665370.22117278430355194No Hit
NGGAGGGA1472470.19555431507920232No Hit
CGGAGGAA1459150.19378532591347739No Hit
ACAGAAAT1293610.17180045605656272No Hit
NTCTCTCG1248600.16582281323754777No Hit
NATCTCTC1219520.16196078583970389No Hit
GTCTCGCG1159650.15400963108355142No Hit
AACCTCTC1151830.15297108038715734No Hit
GTCACTCG1124560.14934943364922054RNA PCR Primer, Index 47 (100% over 8bp)
AAACTCTC1078350.14321242243689705TruSeq Adapter, Index 19 (100% over 8bp)
GTCTCTCA931220.12367252934732441No Hit
CGGAGGGC858350.11399488366366263No Hit
GTCGCTCG843990.11208777522373696No Hit
AATCTCTA785550.1043265344696105No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)