FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005709005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79822937
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGCTC2195005527.49843068290008No Hit
TCGATACT1792711322.458598585516842No Hit
GTTGAGAA1704675621.355711328937947No Hit
AGATCTGG1578328319.772866788903045No Hit
CACCGCCC17624792.207985657054939No Hit
TCGATACC4415620.553176839383898No Hit
CACCGCGC3865160.48421671079328993No Hit
CACCGCAC3858220.48334728650738573No Hit
AGACCTGG3182500.3986949264971295No Hit
AGAACTGG2822280.35356754663136486No Hit
AGATCCGG2804080.35128750023317235RNA PCR Primer, Index 30 (100% over 8bp)
GCTGAGAA2583150.3236099919500582No Hit
NACCGCTC1843640.2309661945914117No Hit
AGAGCTGG1766810.22134114158189896No Hit
GGTGAGAA1693130.21211071198745796No Hit
GTCGAGAA1644220.20598340048550204No Hit
TCGATACA1575900.1974244570830562No Hit
NCGATACT1533190.19207386468378132No Hit
TCGATACG1514670.18975372955770847No Hit
NTTGAGAA1466270.1836903094658118No Hit
TCGAAACT1405710.17610351771446345No Hit
AGATCTGA1337950.16761472958580814Illumina PCR Primer Index 9 (100% over 8bp)
NGATCTGG1333650.1670760373049165No Hit
CCGATACT1288620.16143480162850934No Hit
CACCGCTA1174980.14719829214001484No Hit
GATGAGAA1013160.1269259235600414No Hit
AACCGCTC964600.1208424591042046Illumina Paired End Adapter 2 (100% over 8bp)
TCGACACT949210.11891444184771102No Hit
TCGAGACT872250.10927310279249686No Hit
AGATCGGG802070.10048114365924672No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)