FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78047374
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCGC2018233525.85908271558246No Hit
TCTCGTTT1771550422.698398539328178No Hit
CAAGTAAA1732955422.203891190496687No Hit
GGCTAGCG1668570521.378944793197014No Hit
GGCCAGCG7801080.9995313871803042No Hit
CAAGCAAA4648710.5956267023154425No Hit
TCCCGTTT3478460.44568571903521065No Hit
ACGACCGC3144890.40294629259403403No Hit
GGCAAGCG2920140.37414967991107556No Hit
CAAGGAAA2828270.36237862403929183No Hit
CAAGAAAA2774170.35544693662595234No Hit
GGCGAGCG2307400.29564095263474205No Hit
TCTCGCTT2205570.2825937487659739No Hit
TCTCGTCT2065510.2646482378766517No Hit
TCTCGGTT2028070.25985115143015575No Hit
TCTCGTTA1917170.24564183286935443No Hit
NTGACCGC1785320.22874824718638198No Hit
TCTCGTGT1568970.20102790389847067No Hit
NAAGTAAA1526220.19555046144153423No Hit
AAGACCGC1497760.19190395822926726No Hit
NCTCGTTT1469350.18826386138244702No Hit
NGCTAGCG1459290.1869749006545691No Hit
AGGACCGC1183760.1516719832239327No Hit
ATGACCCC897880.11504294814582744No Hit
TCTCGTAT868350.11125934871300092Illumina Paired End PCR Primer 2 (100% over 8bp)
GGCTAGCA797780.10221740452151534No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)