FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79856492
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGCGC2318044329.027624955025573No Hit
CTTATGAG1801856522.563682111155096No Hit
GAGGATCT1750351421.918711380409746No Hit
AGACCATA1470499818.41427995609925No Hit
TCCCGCGC5522340.6915330064836808No Hit
AGACCACA4362670.5463137549292798No Hit
CCCTGCGC3535920.44278428859609814No Hit
GAGGATCC3210200.40199612074119156No Hit
AGACCAAA3188650.39929752987396444No Hit
GAGGACCT2920310.3656947515300321No Hit
CCTATGAG2912230.36468293648561473No Hit
GAGGAACT2623020.32846672002571814No Hit
CTCATGAG2304760.2886127279420188No Hit
AGACCAGA2127970.2664742648600191No Hit
NCCTGCGC2093270.26212897005292946No Hit
ACCTGCGC2002620.2507773569617859No Hit
CTTACGAG1924280.2409672591177684No Hit
TCCGGCGC1861440.23309814310400712No Hit
GAGGAGCT1805840.2261356534419268No Hit
GCCTGCGC1565610.19605293956563982No Hit
NTTATGAG1538910.19270944183223074No Hit
NAGGATCT1483120.1857231594896505No Hit
NGACCATA1290190.1615635708115002No Hit
CGTATGAG1244980.1559021650988626No Hit
GAGGATCG1216820.1523758393995068No Hit
CATATGAG1074310.13453007677822865No Hit
GAGGATCA969530.12140903960569668No Hit
CTAATGAG832910.10430085007991587No Hit
AGACTATA812490.10174376304934607No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)