FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77130511
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAGAA1938468325.132315018631214No Hit
CGAACCTG1825966523.673724915422902No Hit
ATCTTACC1707450222.137156591637257No Hit
TATGGTGT1696681221.99753609826337No Hit
CGAACCCG7136600.9252628962875663No Hit
CGAACCGG2992140.38793208565673837No Hit
CGAACCAG2772300.3594297462906735No Hit
TATGGCGT2590030.33579837167162035No Hit
ACCTTACC2189620.2838850633311634No Hit
ATCCTACC1953300.2532460857156774No Hit
TATGGGGT1822020.23622558393266704No Hit
NCGCAGAA1724660.22360282301254297No Hit
NGAACCTG1592300.20644229881998316No Hit
AGCTTACC1529300.19827432492959887No Hit
NTCTTACC1471760.19081424210971454No Hit
NATGGTGT1456640.1888539283760223No Hit
ATCGTACC1400020.1815131239050134No Hit
ATCTCACC1388120.17997028439238527No Hit
CGAACCTA1278650.1657774573799984No Hit
TATGGTGC1248900.16192035859842807No Hit
CATGGTGT1193200.15469883247629462No Hit
ATCTTACA1115320.14460166094322907No Hit
GATGGTGT1073990.13924321077037854No Hit
TATGGTGG1062580.1377638999435645No Hit
AACTTACC1045580.13555984349695285No Hit
GCGCAGCA1027020.1331535324587698No Hit
TATGGAGT861250.11166138909672203No Hit
ATCATACC839630.1088583478981489No Hit
TATGGTGA795340.10311613260282951No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)