FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13102455
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2242341.711389201489339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT976570.7453336035117083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT869130.6633337034929713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288220.21997404303239354No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217760.16619786139315113No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163860.12506053254905283No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC163600.12486209645444309No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156920.1197638152544695No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAA154730.1180923727652566No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT148700.11349018180180737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA235550.036.5505071
GTATCAA716450.030.2136121
TATCAAC962100.022.520282
ATCAACG1017050.021.2563363
TCAACGC1032750.020.9327964
CAACGCA1048450.020.6279225
AACGCAG1075550.020.131266
ACGCAGA1193850.018.132667
CGCAGAG1196850.018.083458
AGAGTAC1239050.017.449411
GCAGAGT1242950.017.3910279
CAGAGTA1248200.017.32328810
TCTGTCG21600.017.1860528
ACGATCG1205.0863873E-616.9078391
GAGTACT734650.016.51565712
AGTACTT741050.016.3638513
CGTCGTA20150.016.18970110
CGATCGT1004.1733574E-415.7489123
GTCTCGC19400.015.6876851
TACCGTC21600.015.6236837
Produced by FastQC (version 0.11.5)