FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15925899
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2475141.554160302033813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1082540.679735567831995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT981420.6162415069943619No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327850.20585965037201354No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC281040.17646727509699767No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAA274660.17246122181234477No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT271170.1702698227585143No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245100.15390026019881203No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACA236500.14850025106902914No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179630.11279112092824398No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAG165890.10416366448135833No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164340.10319040702192071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA277750.037.3380781
GTATCAA862800.030.5943361
TATCAAC1169500.022.583142
ATCAACG1228900.021.4419733
TCAACGC1252900.021.0433484
CAACGCA1273400.020.6886795
AACGCAG1315800.020.0631736
CGCAGAG1468550.017.9609978
ACGCAGA1471250.017.9463887
AGAGTAC1511200.017.4197411
GCAGAGT1518050.017.344219
CAGAGTA1520400.017.32321210
GAGTACT829950.016.34308412
AGTACTT837450.016.20199213
CGTCGTA28450.016.05293510
TACCGTC30900.015.7267387
AGTACAT815600.015.6457713
ACCGTCG30200.015.569798
ATACCGT32150.015.3951926
GAGTACA834200.015.3455612
Produced by FastQC (version 0.11.5)