FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13356558
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2204941.6508295026308424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT973570.7289078518582407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT891540.6674923284876239No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287850.2155121102307945No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215420.16128406734729112No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC195250.146182871365512No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAA188770.14133132203671034No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT167240.12521189965259014No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153140.11465528768714216No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACA148040.11083693867836308No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141580.10600036326724295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA246300.037.992181
GTATCAA750200.030.3266621
TATCAAC1011600.022.4822432
ATCAACG1063350.021.3613263
TCAACGC1083650.020.9709954
CAACGCA1102200.020.6058065
AACGCAG1129650.020.1291456
ACGCAGA1263350.018.0042257
CGCAGAG1266400.017.9590878
AGAGTAC1307200.017.37604711
CAGAGTA1317650.017.24336410
GCAGAGT1318150.017.228429
GAGTACT740350.016.70445612
CGTCGTA19450.016.65660910
TACGACG23650.016.647565
AGTACTT746850.016.56497613
ACCGTCG20100.016.4538768
TACCGTC21550.016.181977
CGACGGT26350.015.8810537
CCGTCGT20700.015.7595819
Produced by FastQC (version 0.11.5)