FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17396831
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2645561.5207137437847158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1239660.7125780551641847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1103000.6340235184212573No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346450.19914546505625078No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261030.15004456846192274No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC236560.13597878832070048No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAA221750.12746574361732893No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181580.10437533134626646No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT177150.10182889056058542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA310150.038.2865871
GTATCAA948500.030.530431
TATCAAC1299100.022.2858832
ATCAACG1373800.021.018123
TCAACGC1399300.020.6393324
CAACGCA1421700.020.2983175
AACGCAG1468400.019.686646
CGCAGAG1628550.017.7368078
ACGCAGA1631250.017.7247
AGAGTAC1667100.017.30357211
CAGAGTA1679450.017.18704410
GCAGAGT1680500.017.1737779
GAGTACT876000.016.50871812
AGTACTT884850.016.35363413
CGTCGTA32850.016.02588810
ACCGTCG34250.015.9621388
TACGACG35600.015.9255125
ATACCGT36050.015.8516726
TACCGTC36200.015.7237937
AGTACAT927900.015.46397813
Produced by FastQC (version 0.11.5)