FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14801020
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1844661.2463059978298794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT809370.5468339344180334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT723090.4885406546305592No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245000.16552913245168238No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175150.11833643897515173No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC169600.11458669740328709No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT165610.1118909372462168No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAA164830.1113639465388196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213750.035.7016871
GTATCAA654850.030.3505081
TATCAAC884500.022.46632
ATCAACG924600.021.4254043
TCAACGC940750.021.0692634
CAACGCA951900.020.7870185
AACGCAG976600.020.2890576
ACGCAGA1090750.018.1575017
CGCAGAG1099250.018.0170978
AGAGTAC1125150.017.56624811
CAGAGTA1132850.017.46075210
GCAGAGT1134400.017.4191639
GAGTACT617950.016.43447512
ATACCGT31600.016.3753786
AGTACTT623950.016.26190813
ACCGTCG29200.016.1803198
TACCGTC31050.016.0857757
CGTCGTA29250.015.76796510
AGTACAT623500.015.42207413
GAGTACA634750.015.18074212
Produced by FastQC (version 0.11.5)