FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12171128
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1436141.179956368875588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT641060.5267054951685661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT561180.46107476644728407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199770.16413433496057225No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT191140.1570437842737337No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAA172550.1417699329100803No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC169990.13966659458350944No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACA164430.1350984066554883No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139340.11448404782202604No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT136700.11231498017274981No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC128270.10538875279267461No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACA125410.10303892950595868No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC123640.10158466824110304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177950.034.4484631
GTATCAA514050.029.6351531
TATCAAC700900.021.7664592
ATCAACG727800.020.8913
TCAACGC740100.020.5463374
CAACGCA751700.020.226285
AACGCAG774800.019.6493856
ACCGTCG33250.018.0668
TACCGTC34850.017.6239097
ATACCGT35550.017.466746
ACGCAGA872100.017.4545277
CGTCGTA33450.017.41991610
CGCAGAG874800.017.3877988
AGAGTAC896700.016.91295611
CAGAGTA904800.016.76652110
GCAGAGT905850.016.7346699
CCGTCGT35750.016.4250569
GAGTACT477300.015.63494912
GTCGTAG37350.015.54073211
AGTACTT482050.015.49488613
Produced by FastQC (version 0.11.5)