FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14806235
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970822.006465519424756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1652231.1159015104109855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1393060.9408603875326847No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392370.26500322330423637No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297370.20084106459204518No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210800.14237245322663053No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAA196940.133011531966094No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC192490.1300060413737861No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171150.11559319435359494No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166340.11234456294932507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA347850.038.963431
GTATCAA999050.029.3895471
TATCAAC1420800.020.6633592
ATCAACG1485550.019.7471143
TCAACGC1514050.019.377984
CAACGCA1533550.019.1125055
AACGCAG1574950.018.6345126
CGTCGTA29250.017.84431610
ACCGTCG30550.017.4532538
ATACCGT32950.017.0696776
TACCGTC32100.017.0310137
ACGCAGA1745150.016.8196587
CGCAGAG1745350.016.806138
CCGTCGT31600.016.5885379
AGAGTAC1789550.016.35829511
CAGAGTA1799850.016.2684310
GCAGAGT1800700.016.2645559
GTCGTAG33300.016.01186411
GAGTACT1049350.015.84818112
AGTACTT1052100.015.81306413
Produced by FastQC (version 0.11.5)