FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13017774
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1773491.3623604158437532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT786940.6045119541943192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT707830.5437411956913678No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245980.1889570367406901No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168030.12907736760524496No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139210.1069384059056487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205550.036.1660081
GTATCAA628850.030.3908621
TATCAAC849400.022.4787062
ATCAACG894400.021.2884883
TCAACGC909000.020.9537454
CAACGCA921200.020.6591455
AACGCAG946350.020.1291336
CGCAGAG1059450.017.976038
ACGCAGA1059350.017.9756037
CGTACCG901.8687051E-417.4985795
AGAGTAC1092500.017.41760811
GCAGAGT1106100.017.2178869
CAGAGTA1107400.017.20966510
GAGTACT592700.016.34106812
AGTACTT603050.016.06434213
TACGACG18700.015.5201315
CGTCGTA16200.015.41523710
AGTACAT604450.015.40927613
GAGTACA623450.015.02988512
TCTGTCG18600.014.9987828
Produced by FastQC (version 0.11.5)