FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14076075
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2528291.7961612168164776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1097180.7794644458771355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010350.717778215873388No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327960.23299108593837414No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239100.16986269254746086No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC201570.14320043051774023No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAA200020.14209927128123429No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT189580.13468243100438154No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175090.12438836820633592No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163360.11605507927458471No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACA158560.11264503776798575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA278600.037.73281
GTATCAA875950.030.7117841
TATCAAC1177750.022.824642
ATCAACG1241700.021.610713
TCAACGC1263700.021.2430884
CAACGCA1282100.020.9118945
AACGCAG1316600.020.3998786
ACGCAGA1465900.018.3145057
CGCAGAG1466650.018.3005378
AGAGTAC1507750.017.75678811
CAGAGTA1516050.017.66996210
GCAGAGT1518400.017.6323689
GAGTACT831800.016.9750412
AGTACTT839050.016.83634813
AGTACAT804250.015.70738813
TACGACG26750.015.3072065
GAGTACA829200.015.30264912
GTACTTT927500.015.24289714
CGACGGT28950.014.8434447
TACTTTT953600.014.84226715
Produced by FastQC (version 0.11.5)