FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9495803
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1368661.4413315019277464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT604150.6362284474519954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT534060.56241689091486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188550.19856140654981994No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138890.14626461816867936No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT112350.11831542840558086No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111600.11752560578605095No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAA107540.11325003267232901No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC103060.10853215889167035No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99300.10457251482576038No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACA96860.10200295857022308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136400.037.4393421
GTATCAA431800.030.712611
TATCAAC588900.022.441732
ATCAACG616950.021.362123
TCAACGC627700.021.006914
CAACGCA637450.020.6785475
AACGCAG655950.020.1194576
ACGCAGA733600.017.9898557
CGCAGAG735550.017.9513388
TACCGTC17000.017.6014167
CGTCGTA15300.017.35143910
AGAGTAC762450.017.2943911
GCAGAGT766250.017.2144979
CAGAGTA769450.017.15167610
ATACCGT18150.017.1059576
TACGACG18250.016.6423055
ACCGTCG17350.016.3386448
GAGTACT461100.016.03797312
AGTACTT464800.015.8908613
TCTAGCG19600.015.840631528
Produced by FastQC (version 0.11.5)