FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23938068
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC57100.024.50667845
CTCCGAG95200.014.67498244
TCTCCGA105600.013.16583343
ATCTCCG145850.011.73838745
TATACAC975000.011.359445
ATACACA782450.011.16741945
TACACAT539800.09.85246245
TATACTG59950.09.642265
TTATACA1213100.09.17991745
ACACATC426150.09.032701545
TACACTG73300.08.9601195
GTCCTAG50350.08.7408031
TAGTACG5952.910383E-118.6948144
GTATAGA43200.08.603941
GTGTTAG57800.08.5610181
GTGTATA77800.08.3240031
TACACCG8400.08.300755
GTGTAGG47400.08.2264281
GTCTTAG44900.08.1766051
TAGACAG87400.08.1322475
Produced by FastQC (version 0.11.5)