FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22856479
Sequences flagged as poor quality0
Sequence length25-355
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG422150.1846959892641382No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT345340.15109063823872435No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT342720.14994435494635897No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC333180.14577048372148657No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC330170.14445357047338744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCC49552.7307484E-512293.222340-344
GGGTTAA24051.0534811E-48442.538340-344
ATTTCCG50101.0390206E-48105.5107330-339
AATCCGA39551.5539741E-46845.109310-319
AAATGGG123602.5295315E-44928.229340-344
AATGGGT74053.1776546E-44700.5234340-344
TTTGGGG162851.7118145E-74274.7773320-329
AAATTTC152800.03986.4473340-344
ACCCGGG102100.03977.3367330-339
TGGGTTA58200.03805.8677340-344
TCCCCCG72400.03739.2825310-319
TAAACCG13706.6031754E-43487.2139290-299
ATCCGAA46208.0098026E-43102.2617310-319
CCCGGGA133051.8189894E-123052.131330-339
AACCCCC72709.625153E-42792.8892330-339
TTTCCGG76000.001051872671.619330-339
GCCCTTT151000.02482.4417320-329
GGGCCAA110350.00120958572453.3215310-319
ATGGGTT102050.00129307212387.5713340-344
GGGTTTT148001.8189894E-122351.8499340-344
Produced by FastQC (version 0.11.5)