FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23153957
Sequences flagged as poor quality0
Sequence length25-364
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG386300.1668397328370265No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT380800.16446432892658477No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT369250.1594759807146571No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC365000.15764044132931576No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC348480.15050559176558892No Hit
CTCATGGTTCACACCCATGACGAACATGGGGGCGTCAGCAGAGGGGGCAG269080.11621339713121175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA12954.588046E-637794.082290-294
TGATCGA65550.037332.83305
GCCGTGA63751.1118678E-519193.465300-304
GGCCGTG179650.013621.858300-304
CCGTGAT60250.013538.959300-304
CGCGATC18400.010230.631290-294
GTGATCG55550.08810.682300-304
GCGCGAT35001.20643075E-47768.7837290-294
GCGATCG23450.06138.635290-294
TCGCGCG13053.8200364E-44573.716285-289
CCCGGGT109700.04461.562290-294
TAAACCC47404.056368E-44302.3325285-289
TCCGGCG64604.1096692E-44209.0933290-294
TACTTAT22504.5006713E-44183.191275-279
CGATCGA28700.04060.34290-294
CGTGATC152150.04020.9773300-304
GCGCTGG206703.5064731E-43946.4067295-299
GCGGGAT59606.412963E-43421.6538285-289
ATCCGGC148156.004039E-43303.6338290-294
ATTCCGC41400.02955.5156280-284
Produced by FastQC (version 0.11.5)