FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21630736
Sequences flagged as poor quality0
Sequence length25-355
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT290150.13413783053891462No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC275940.12756847478513905No Hit
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG260640.12049520645067278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGG140300.016664.803340-347
CGGGTTT67104.0482024E-511614.863330-339
TGTTCGC29450.011341.6045300-309
GGGTTTA44658.962235E-58727.405330-339
CCCGGGG152801.8291757E-87650.7583340-347
TAAACCC47507.6917786E-86152.821330-339
CTAGCAC57700.05788.7393300-309
TTTAAAA138301.7197002E-45635.266320-329
TTTCCCG71802.317442E-45427.279320-329
TTAAACC64901.2752207E-75146.537330-339
TTAAAAC75952.5930654E-45130.726320-329
CGATCAA33050.05053.105290-299
AAATTTC155500.05011.944330-339
GGTTTAA62553.2829965E-44672.406330-339
CTTACCT71500.04671.472300-309
CCTTTTA84353.198335E-44619.782330-339
CCCTTTT169500.04597.978330-339
TTTTCCC173152.69556E-44501.0527320-329
CCGGGGT89350.04361.2607340-347
TTTAAAC90950.04284.5366330-339
Produced by FastQC (version 0.11.5)