FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21636341
Sequences flagged as poor quality0
Sequence length25-354
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG401650.1856367488384473No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT367930.17005185858366717No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC352760.1630405067104461No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT341570.15786865256006088No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC319440.14764049059866452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT99950.022973.475300-309
CCCAAAT106000.021662.252330-339
GTTTAAA108150.021231.611290-299
CCAAATT98353.005728E-511673.608330-339
CCCTTTT215250.010667.591310-319
TTAAAAC83306.468472E-59188.471290-299
TTTAAAA139856.0775128E-58209.506290-299
CGCTTGG64701.3007237E-47097.9873280-289
GGGGTTT176250.06514.039300-309
AACCCGG52900.06200.9146290-299
CCGGGGA380650.06032.31340
AAGGGGG97050.05914.9893320-329
GGGTTTT137601.764988E-45562.497300-309
TAAAACC104502.0359096E-45493.2983290-299
CAAATTT145751.9802545E-45251.4546330-339
CTAATCA25902.834591E-45215.0776270-279
GGAAAGG482150.04762.416320-329
CCTTTTT242250.04739.3164310-319
AAACCCG91103.8679695E-44200.876290-299
TTTCCCG79104.0824732E-44147.009330-339
Produced by FastQC (version 0.11.5)