FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19797559
Sequences flagged as poor quality0
Sequence length25-365
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG236430.1194238138146223No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT229220.11578195069402242No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC214600.10839720189746624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT86050.024468.57310-319
CCCCAAA166850.012619.241340-347
TAAACCG10905.2688316E-512072.9375300-309
CCTTTTA66254.8661437E-510593.814300-309
GGAAAAG230700.09126.66330-339
CCCTTTT146250.07198.3604320-329
CCCAAAA154250.06825.0254340-347
CTGTTAT42050.06258.9785290-299
CCAAAAT129700.05411.258340-347
CTTTTAA67352.514377E-45210.3945300-309
CGGGGTT209000.05037.1304310-319
GGTTTTA72252.893532E-44857.025300-309
AAAAGGG145352.3422454E-44828.6216330-339
GAAAAGG236700.04447.6562330-339
CCCCTTT480400.04382.848320-329
CCTTTTG160752.8648518E-44366.0347320-329
ATTTACG8504.7254632E-44128.471270-279
AAGGGGC105154.0857913E-44004.794330-339
TTAAACC76602.8707655E-73926.7441300-309
AAAATTT109204.406576E-43856.2646340-347
Produced by FastQC (version 0.11.5)