FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19972804
Sequences flagged as poor quality0
Sequence length25-357
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG308570.15449508241306528No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT286720.1435552063696214No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT283010.141697680505952No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC266730.13354659666214116No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC257670.1290104283805118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT17552.4096873E-517070.064285-289
GTTTAAA104300.010053.008300-304
AGTGACT70751.1188809E-47410.097300-304
TAAACCC41201.3279587E-47271.3496300-304
GGGTTTA38201.5221325E-46862.0986295-299
TTGGGGA164901.01307276E-46358.573295-299
TTGGGTT97950.05352.3667295-299
TAACGAG9352.940745E-45215.9116260-264
TTTAAAC101100.05185.602300-304
TTAAACC70550.04954.069300-304
TTTGGGT217400.04823.039295-299
GGTAGCA62553.0607654E-44789.4424285-289
GAGTGAC283900.03693.303300-304
AATCGAC15500.03146.3728270-274
GGTTTAA69350.03023.8752295-299
GGAAATT145100.02890.4993290-294
AATTCCG30000.00100569862796.0764280-284
GAATCGA34150.02791.2385270-274
CGCAATT19200.02663.9448275-279
TGGGGAG197458.7138545E-42655.1753295-299
Produced by FastQC (version 0.11.5)