FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21693873
Sequences flagged as poor quality0
Sequence length25-364
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT616540.2842000596205205No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC590380.27214135530340755No Hit
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG462430.21316156870651912No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT446570.20585074873444684No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC412150.18998451774839836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCC45950.049344.0340-341
TTGGGGG132800.017073.469310-319
GGGGGTT61150.012359.536310-319
TCGGAAT52155.2002804E-510869.4290-299
AAACCCG105100.010786.664340-341
GGGTTTA43656.0720027E-510388.806310-319
AGGGCCC226200.010023.682330-339
TGGGGGT123504.8608712E-59179.582310-319
GGTTTAA46351.0269452E-48153.0273310-319
AAACCCC310500.07302.277320-329
CTGATTT156357.79069E-57250.9014300-309
TAACGGG14002.431807E-86229.0024280-289
GTAACGG28752.3969184E-45633.1846280-289
TGATTTG102200.05546.372300-309
CCCTTTA75352.7139354E-45015.1665330-339
GCCCCTT113352.456663E-45000.7866330-339
AACCCCA230150.04925.824320-329
TTAAACC46353.0806675E-44891.8164310-319
GGGCCCC233301.7346414E-44859.316330-339
CGGAATC60303.2443422E-44700.159290-299
Produced by FastQC (version 0.11.5)