FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29642028
Sequences flagged as poor quality0
Sequence length25-359
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG656330.2214187234422692No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT618680.20871716334658347No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT598000.20174058266188805No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC573490.1934719176434217No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC564800.19054026937698054No Hit
CCATCGTCGGCGCCCCAGACACCAGGGCGTCATGGTGGGCATGGGCCAGA370320.12493072336346217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG10255.1505594E-7102972.66330-339
TACCGTC38900.040699.348340-349
TAACGGG28151.9423325E-518747.242330-339
ACCGTCC71150.014834.431340-349
GGGTTTA62203.793207E-512726.725310-319
TGGACCA283400.011172.933350-351
GGTTTTT143503.3650354E-511032.785340-349
GGACCAA163804.3844353E-59665.474350-351
TCGGGAA101801.0160476E-47776.0537300-309
CCGTCCC103600.07640.9487340-349
TAAAACC143451.0087769E-47357.7534330-339
CGTCCCT87101.2396477E-47270.744340-349
TAAACCC65101.4542442E-46948.4517310-319
CCGGGGG155851.1907113E-46772.3433320-329
TTAAACG30651.8419969E-46456.7886280-289
GGCCCAA131350.06026.664310-319
ACCGGGT70850.05586.4663290-299
CGGGGGG98552.3804238E-45354.996320-329
GGTTTAA95153.1065565E-44754.011310-319
AAAATTT169702.8233827E-44664.716310-319
Produced by FastQC (version 0.11.5)