FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18776554
Sequences flagged as poor quality0
Sequence length25-365
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG481020.25618119277903706No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT448900.2390747524812061No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC406520.21650405074328335No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT263750.1404677343883228No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC254460.13552007466332747No Hit
CCATCGTCGGCGCCCCAGACACCAGGGCGTCATGGTGGGCATGGGCCAGA235160.12524129827017247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCC44550.045364.05320-329
GTTAACC23400.028788.725300-309
ACCCGGG88750.022771.475310-319
TTAACCC22203.6379788E-1215172.437300-309
GTTTCCC153100.013200.315330-338
CCGTCGT29050.011594.77290-299
CCGGGGT75350.08940.359310-319
CCCGGGG123306.0985567E-58195.33310-319
GGGTTTA43701.1490263E-47707.736310-319
CCGGAAT46301.2898156E-47274.904290-299
GGTTTTT94051.0644524E-47162.745300-309
ACCCCGG73851.3125989E-46841.4634320-329
CGTCGTT24751.6216599E-46804.6074290-299
CCGGGTT56800.05082.9185320-329
TTTCCCT203851.6669491E-44956.9985330-338
GCCCTTT137600.04895.757300-309
AACCCCG143802.488395E-44684.6743320-329
GGTTTAA61753.2118105E-44675.4614310-319
TAACCCA50853.7336562E-44415.969300-309
CGGAATT38503.9238384E-44374.3906290-299
Produced by FastQC (version 0.11.5)