FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21545410
Sequences flagged as poor quality0
Sequence length25-362
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG375710.17438052930995512No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT310890.14429523504078132No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT301090.1397467024298911No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC290750.13494753638942122No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC290380.1347758060765611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTTA49550.07677.7583310-319
AACCGGG54001.3753481E-47045.0547320-329
TTAAACC74801.759284E-46103.2017330-339
TAAACCC43302.652807E-45271.588330-339
GTAAACC54602.6245991E-45225.727300-309
CCCGGGG161803.9914084E-74702.5083340-343
GGAAAAA166752.6229685E-44562.9136340-343
ACCGGGT48500.03921.989320-329
GAAAAAT147500.03868.8096340-343
GGTTTTA101754.882808E-43738.8987310-319
CGGAAAT47400.03439.719290-299
AAAATTC116704.87762E-83259.9224340-343
TTTAAAA142106.348854E-43212.6636330-339
TAGGGTA11659.4342144E-42924.3452270-279
TTAACCG14609.589816E-42895.2278310-319
GTTTTCC170659.156065E-42675.1804330-339
TAAACCA57700.00109902352637.317300-309
TTTTCCC177009.1633956E-72579.2063330-339
CCCGTCG24053.640962E-72433.6025280-289
TTGCGTC26006.166616E-62251.0825280-289
Produced by FastQC (version 0.11.5)